Sandro Cortese ended Friday's Second Free Practice session at the top of the timesheets on a windy afternoon in Jerez.
The German put in a series of blistering laps on his final run to set a time of 1:48.711, putting him half a second clear of Nico Terol.
For the first time so far this season, the Bankia Aspar rider didn't finish a timed session on top, as he couldn't match the pace of Cortese.
Teammate Hector Faubel finished third but couldn't get within a second of the fastest time, although he looked much more comfortable than he had in the morning session.
Luis Salom was fourth as he looked to build on a strong weekend in Qatar. The Spaniard was the last man to go under 1:50 as the riders struggled to match this morning's times.
Jonas Folger was 1.316 seconds down on fellow countryman Cortese as he took fifth, as he had in the first session. Johann Zarco was the fastest Derbi in sixth after a troubled session for Efren Vazquez.
The Spaniard, who was third in the morning, suffered a fall early in the session and was unable to rejoin, and his time of 1:52.153 was only good enough for 19th.
He wasn't the only person to hit problems, with the high speed turn 11 catching a number of riders out.
Zulfahmi Khairuddin was the first to fall as he suffered a spectacular accident, sending him tumbling through the gravel trap. Minutes later, he was followed by Niklas Ajo, Hiroki Ono and Sarath Kumar, who all crashed within seconds of each other. All riders seemed to escape injury.
Taylor Mackenzie completed an excellent day at Jerez with the 13th fastest time as the rookie did what few other riders did, and improved on his FP1 time.
That placed him higher than fellow Brit Danny Kent, who didn't set a competitive time until the final few minutes, before climbing to 16th. With the windy conditions making life difficult, his morning time was still good enough for tenth in the combined classification.
Danny Webb had a quiet afternoon as he finished 22nd, just ahead of his teammate Marcel Schrotter, while Harry Stafford was 25th.
