Stefan Bradl led the way in the 20 minute warm-up session ahead of Sunday's Moto2 Grand Prix of Qatar.
The pattern from the weekend continued on Saturday night with Bradl and Spanish star Marc Marquez again dominating the session. The Team Catalunya Caixa Repsol rider improved lap after lap on his final run but couldn't quite overhaul the German, whose time of 2:00.600 remained unbeaten.
Marquez finished a quarter of a second behind and will fancy his chances of challenging for victory in his first ever Moto2 race.
Alex de Angelis grabbed third after improving on his qualifying time and was the only rider within a second of pacesetter Bradl.
Julian Simon followed up his strong qualifying performance by taking fifth in the warm-up, with Aleix Espargaro completing the top six. Pons HP 40 rider Axel Pons sprung a surprise by setting the seventh fastest time after being largely anonymous through the weekend's running. The Spaniard finished two places behind teammate his teammate in seventh.
Scott Redding was 1.624 seconds off the pace in 11th and will be eying a strong points finish from the fourth row in the race, while Bradley Smith couldn't match his Qualifying heroics.
After taking a stunning seventh place earlier on, the Tech 3 rider suffered his first accident in Moto2 but emerged unharmed. He ended the session in 23rd, three places ahead of fellow Brit Kev Coghlan.
Mattia Pasini also had an off-track excursion midway through the session. The Italian was also uninjured and will take start the race, as planned, from 13th on the grid.
The opening race of the new Moto2 season will get underway at the Losail International Circuit, at 17:15 GMT on Sunday.